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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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High Performance Thin Layer Chromatography Method for Simultaneous Estimation of Cefepime Hydrochloride and Sulbactam Sodium
Laxman M. Prajapati
Jimish R. Patel
Amit K. Joshi
Laxman M. Prajapati*, Anjali Patel, Jimish R. Patel, Amit K. Joshi, Mohammadali Kharodiya
Department of Pharmaceutical Chemistry, Shri B M Shah College of Pharmaceutical Education and Research, College Campus, Modasa-383315, Gujarat, India.
A Simple High Performance Thin Layer Chromatography method for the simultaneous estimation of cefepime hydrochloride and sulbactam sodium was developed. The determination was carried on Silica Gel 60 GF254 HPTLC Plates using the mobile phase of chloroform:ethyl alcohol: Diethyl amine: water (12:7:1:0.4V/V). The absorbances of the spots were measured by densitometry at 254nm. The Retention Factor (Rf) was found to be 0.17 for cefepime hydrochloride and 0.76 for sulbactam sodium respectively. Cefepime hydrochloride and sulbactam sodium showed linear response at concentration range 4-20 µg/band and 2-10 µg/band repepectively. The Correlation co-efficient (r2) for cefepime hydrochloride and sulbactam sodium was found to be 0.9997 and 0.9997 respectively. The percentage recoveries obtained for cefepime hydrochloride and sulbactam sodium in range form 99.87-100.12 and 98.91-100.50.
Combined dosage, HPTLC, cefepime hydrochloride, sulbactam sodium, validation.
Laxman M. Prajapati, Anjali Patel, Jimish R. Patel, Amit K. Joshi, Mohammadali Kharodiya. High Performance Thin Layer Chromatography Method for Simultaneous Estimation of Cefepime Hydrochloride and Sulbactam Sodium. Asian J. Pharm. Ana. 2016; 6(4): 207-212..
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