Volume No. :   6

Issue No. :  3

Year :  2016

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Validated HPLC-UV Method for Simultaneous Determination of Some Anti-Inflammatory and Analgesic Drugs



Address:   Hamid Khan1*, Mushir Ali, Alka Ahuja, Javed Ali2
1JK College of Pharmacy, Bilaspur, CG, India-495001
2Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, New Delhi, India-110062
*Corresponding Author
DOI No:

ABSTRACT:
In the presented work identification and quantification of some anti-inflammatory and analgesic drugs, namely aceclofenac, diclofenac, paracetamol and para-aminophenol were carried out by validated HPLC-UV method. The chromatographic separation was achieved on HPLC C18 (100.0 × 2.1 mm, 1.7µm) column using isocratic mobile phase consisting of acetonitrile-phosphate buffer (50:50, v/v) at a flow rate of 1.0 mL/min. The UV detection was carried out at 275 nm for all the compounds. The elution of aceclofenac, diclofenac, paracetamol and para-aminophenol was occurred at 7.0, 9.2, 2.0 and 4.2 min, respectively. The calibration curves were linear over the concentration range of 1-1000 µg/mL for aceclofenac and paracetamol, and 1-100 µg/mL for diclofenac and para-aminophenol. The developed method was validated according to ICH guidelines. The method was applied in the identification and quantitative determination of these compounds during routine quality control analysis and in stability studies.
KEYWORDS:
HPLC-UV, Aceclofenac, Paracetamol, Diclofenac, Para-aminophenol, Validation.
Cite:
Hamid Khan, Mushir Ali, Alka Ahuja, Javed Ali. Validated HPLC-UV Method for Simultaneous Determination of Some Anti-Inflammatory and Analgesic Drugs. Asian J. Pharm. Ana. 2016; 6(3): 183-187.
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