Volume No. :   6

Issue No. :  2

Year :  2016

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Development and Validation of Analytical Method for Clopidogrel Bisulphate and Irbesartan by Simultaneous Equation Spectroscopic Method



Address:   Pankaj Savani1*, Sudhanshu Chauhan1, Vineet Jain2, Hasumati Raj1, Sagar Patel1
Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Dist: Surat, Gujarat, India. 394110
2Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Dist: Surat, Gujarat, India. 394110
*Corresponding Author
DOI No: 10.5958/2231-5675.2016.00015.6

ABSTRACT:
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Clopidogrel Bisulphate and Irbesartan in synthetic mixture using simultaneous equation Method. In this spectroscopic method, for Clopidogrel Bisulphate 220.00 nm and 250.00 nm wavelengths were selected for measurement of absorptivity. Both the drugs show linearity in a concentration range of 10-50 µg/ml at their respective ?max with correlation coefficient (r2) of 0.9996 and 0.9998 for Clopidogrel Bisulphate and Irbesartan, respectively. Accuracy, precision and recovery studies were done by QC samples covering lower, medium and high concentrations of the linearity range. The relative standard deviation for accuracy, precision studies were found to be within the acceptance range (< 2%). The limit of determination was 0.243 µg/ml and 0.323 µg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. The limit of quantification was 0.737 µg/ml and 0.980 µg/ml for Clopidogrel Bisulphate and Irbesartan, respectively. Recovery of Clopidogrel Bisulphate and Irbesartan were found to be 99.57 % and 99.68 % respectively confirming the accuracy of the proposed method. % Assay was found to be 99.41 % and 99.22 % for Clopidogrel Bisulphate and Irbesartan, respectively. The proposed method is recommended for routine analysis since they are rapid, simple, accurate and also sensitive and specific by no heating and no organic solvent extraction.
KEYWORDS:
Clopidogrel Bisulphate, Irbesartan, simultaneous estimation, Validation method
Cite:
Pankaj Savani, Sudhanshu Chauhan, Vineet Jain, Hasumati Raj, Sagar Patel. Development and Validation of Analytical Method for Clopidogrel Bisulphate and Irbesartan by Simultaneous Equation Spectroscopic Method. Asian J. Pharm. Ana. 2016; 6(2): 102-108.
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