Registered User Login
Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
News & Conferences
Volume No. :
Issue No. :
ISSN Print :
ISSN Online :
Click to Login
Simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture using Spectrophotometric technique (First order Derivative Method)
Pranali S. Sisode
Hasumati A. Raj
Vineet C. Jain
Pranali S. Sisode1 , Hasumati A. Raj1, Vineet C. Jain2
1Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India.
2Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India.
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Saxagliptin Hydrochloride and Glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin Hydrochloride showed zero crossing point at 315.00nm while Glibenclamide showed zero crossing point at 229.40nm. The dA/d? was measured at 229.40nm for Saxagliptin Hydrochloride and 315.00nm for Glibenclamide and calibration curves were plotted as dA/d? versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25µg/ml for Saxagliptin Hydrochloride at 229.40nm. The linear correlation was obtained (r2>0.9994) in the range of 5-25 µg/ml for Glibenclamide at 315.00nm. The limit of determination was 0.243µg/ml and 0.317µg/ml for Saxagliptin Hydrochloride and Glibenclamide, respectively. The limit of quantification was 0.738µg/ml and 0.960µg/ml for Saxagliptin Hydrochloride and Glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in binary mixture.
Saxagliptin Hydrochloride, Glibenclamide, First Derivative Method, Spectroscopic method.
Pranali S. Sisode , Hasumati A. Raj, Vineet C. Jain. Simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture using Spectrophotometric technique (First order Derivative Method). Asian J. Pharm. Ana. 2016; 6(2): 77-82.
Visitor's No. :