Volume No. :   6

Issue No. :  2

Year :  2016

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture using Spectrophotometric technique (First order Derivative Method)



Address:   Pranali S. Sisode1 , Hasumati A. Raj1, Vineet C. Jain2
1Department of Quality Assurance, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India.
2Department of Pharmacognosy, Shree Dhanvantary Pharmacy College, Kim, Surat, Gujarat, India.
*Corresponding Author
DOI No: 10.5958/2231-5675.2016.00012.0

ABSTRACT:
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Saxagliptin Hydrochloride and Glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin Hydrochloride showed zero crossing point at 315.00nm while Glibenclamide showed zero crossing point at 229.40nm. The dA/d? was measured at 229.40nm for Saxagliptin Hydrochloride and 315.00nm for Glibenclamide and calibration curves were plotted as dA/d? versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25µg/ml for Saxagliptin Hydrochloride at 229.40nm. The linear correlation was obtained (r2>0.9994) in the range of 5-25 µg/ml for Glibenclamide at 315.00nm. The limit of determination was 0.243µg/ml and 0.317µg/ml for Saxagliptin Hydrochloride and Glibenclamide, respectively. The limit of quantification was 0.738µg/ml and 0.960µg/ml for Saxagliptin Hydrochloride and Glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in binary mixture.
KEYWORDS:
Saxagliptin Hydrochloride, Glibenclamide, First Derivative Method, Spectroscopic method.
Cite:
Pranali S. Sisode , Hasumati A. Raj, Vineet C. Jain. Simultaneous determination of Saxagliptin Hydrochloride and Glibenclamide in Synthetic Mixture using Spectrophotometric technique (First order Derivative Method). Asian J. Pharm. Ana. 2016; 6(2): 77-82.
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