Volume No. :   6

Issue No. :  1

Year :  2016

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Second Derivative Spectroscopic Method for Simultaneous estimation of Amiodarone Hydrochloride and Ranolazine in synthetic mixture



Address:   Vishakha D. Patel, Hasumati A. Raj, Nirav K. Gheewala
Shree Dhanvantary College of Pharmacy, Kim, Surat, Gujarat, India.
*Corresponding Author
DOI No: 10.5958/2231-5675.2016.00004.1

ABSTRACT:
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Amiodarone Hydrochloride and Ranolazine in synthetic mixture using Second order derivative zero-crossing method. Amiodarone HCl showed zero crossing point at 249.20 nm while Ranolazine showed zero crossing point at 263.20 nm. The dA/d? was measured at 263.20 nm for Amiodarone HCl and 249.20 nm for Ranolazine and calibration curves were plotted as 2dA/2d? versus concentration, respectively The linear correlation was obtained (r2>0.9996) in the range of 1-20 µg/ml for Amiodarone HCl at 263.20 nm. The method was found to be linear (r2>0.9996) in the range of 10-200 µg/ml for Ranolazine 249.20 nm. The limit of determination was 0.235µg/ml and 0.271µg/ml for Amiodarone HCl and Ranolazine, respectively. The limit of quantification was 0.712µg/ml and 0.823µg/ml for Amiodarone HCl and Ranolazine, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows Second order derivation zero crossing. The method was successfully applied for simultaneous determination of Amiodarone HCl and Ranolazine in binary mixture.
KEYWORDS:
Amiodarone HCl, Ranolazine, Second Derivative Method, Spectroscopic method.
Cite:
Vishakha D. Patel, Hasumati A. Raj, Nirav K. Gheewala. Second Derivative Spectroscopic Method for Simultaneous estimation of Amiodarone Hydrochloride and Ranolazine in synthetic mixture. Asian J. Pharm. Ana. 6(1): January- March, 2016; Page 23-30.
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