Volume No. :   4

Issue No. :  3

Year :  2014

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Simultaneous Estimation of Metformin HCl and Pioglitazone HCl by Second Order Derivative UV-Visible Spectrophotometric Method in Tablet Formulation



Address:   Vijaya Vichare*, Pallavi Suryawanshi, Jyoti Bhosale and Shashikant Dhole
PES, Modern College of Pharmacy (for Ladies), Borhadewadi, Dehu-Alandi Road, At Post: Moshi, Pune-412105. Maharashtra, India
*Corresponding Author
DOI No:

ABSTRACT:
The aim of present study was to develop and validate simple, sensitive and accurate second order derivative UV spectrophotometric method for simultaneous estimation of Metformin HCl (MET) and Pioglitazone HCl (PIO) in a bilayer tablet formulation without prior separation by using 0.1 N NaOH as solvent. Since the two drugs are available in very vast differential concentrations; the developed method was based on the standard addition technique. Wavelengths 247.5 nm (Zero crossing for PIO) and 279.5 nm (Zero crossing for MET) were selected for determination of MET and PIO respectively by second order derivative UV spectrophotometric method. The assay results were found to be 98.68% and 99.098 % with SD ±1.51 and 1.22 for MET and PIO respectively. Linearity was observed from 5-30 µg/ ml and 2.5-15 µg/ ml for MET and PIO respectively. Accuracy studies done by % recovery analysis and were found in a range of 96.89-101.197% for MET and 97.38-101.166% for PIO. It shows that method is accurate. Precision of the method was done by inter day and intraday analysis where the % R.S. D. was found to be less than 2, which indicates the validity of method.
KEYWORDS:
Metformine HCl, Pioglitazone HCl, Second order derivative, UV Spectrophotometry.
Cite:
Vijaya Vichare, Pallavi Suryawanshi, Jyoti Bhosale , Shashikant Dhole. Simultaneous Estimation of Metformin HCl and Pioglitazone HCl by Second Order Derivative UV-Visible Spectrophotometric Method in Tablet Formulation. Asian J. Pharm. Ana. 4(3): July-Sept. 2014; Page 121-124.
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