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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Nebivolol and Indapamide in Pharmaceutical Dosage Form
Niraj Vyas* and Sangita Panchal
Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology, At & Post- Changa, Ta- Petlad, Dist. - Anand, 388421, Gujarat, India.
A simple, sensitive, precise and specific Reverse Phase High Performance Liquid Chromatographic method was developed and validated for the determination of Nebivolol and Indapamide in bulk and tablet dosage form. It was found that the excipient in the tablet dosage form does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography by Inertsil ODS C-18 (150 x 4.6 mm) column with a mobile phase composed of Buffer: Acetonitrile (60:40) in isocratic mode at a flow rate of 1ml/min. The detection was monitored at 282 nm. The calibration curve for Nebivolol and Indapamide was linear from 25-225 µg/ml and 7.5-67.5 µg/ml respectively. The inter-day and intra-day precision was found to be within limits. The proposed method has adequate sensitivity, reproducibility and specificity for the determination of Nebivolol and Indapamide.
HPLC, Nebivolol, Indapamide, Validation, Tablet.
Niraj Vyas, Sangita Panchal. Development and Validation of RP-HPLC Method for Simultaneous Estimation of Nebivolol and Indapamide in Pharmaceutical Dosage Form. Asian J. Pharm. Ana. 4(3): July-Sept. 2014; Page 98-102.
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