Volume No. :   4

Issue No. :  3

Year :  2014

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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HPLC Determination of Three Cephalosporins (Cefepime, Cefotriaxone and Cefotaxime) in Their Bulk and Dosage Forms



Address:   Sobhy M. El-Adl1, Abdallah A. El-Shanawany1, Lobna M. Abdel-Aziz1, Ali F. Hassan2*
1Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt.
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assuit, Egypt.
*Corresponding Author
DOI No:

ABSTRACT:
An isocratic HPLC method had been developed for rapid simultaneous separation and determination of three ceohalosporins including Cefepime, Cefotriaxone and Cefotaxime in pure form or in presence of some impurities within 8 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase was studied. Beer’s law was obeyed in the range of 1-70 µg/ml for all drugs. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and was validated when obtained results were compared with reference methods.
KEYWORDS:
Cefepime, Cefotriaxone, Cefotaxime, Hypersil gold, mobile phase.
Cite:
Sobhy M. El-Adl, Abdallah A. El-Shanawany, Lobna M. Abdel-Aziz, Ali F. Hassan. HPLC Determination of Three Cephalosporins (Cefepime, Cefotriaxone and Cefotaxime) in Their Bulk and Dosage Forms. Asian J. Pharm. Ana. 4(3): July-Sept. 2014; Page 91-97.
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