Volume No. :   4

Issue No. :  2

Year :  2014

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract



Address:   A. Suneetha* and K.Manasa
*Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur, A.P - 522002.
*Corresponding Author
DOI No:

ABSTRACT:
A simple, sensitive, precise and accurate UV Spectrophotometric method has been developed for estimation of andrographolide in kalmegh plant extract. The method is based on the formation of red-orange colored complex with picric acid in an alkaline medium and obeys beer’s law in the range of 10 - 100µg/mL exhibiting the maximum absorbance at 481nm. The limit of detection and quantification were found to be 1.0µg/mL and 3.0µg/mL, respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient 0.9998. The Regression equation of the curve was Y=0.0114x + 0.0067. The method was validated according to ICH guidelines.
KEYWORDS:
Andrographolide, Picric acid, NaOH, Validation, ICH guidelines.
Cite:
A. Suneetha, K.Manasa. Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract. Asian J. Pharm. Ana. 4(2): April-June 2014; Page 85-88.
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