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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract
A. Suneetha* and K.Manasa
*Department of Pharmaceutical Analysis, Hindu College of Pharmacy, Guntur, A.P - 522002.
A simple, sensitive, precise and accurate UV Spectrophotometric method has been developed for estimation of andrographolide in kalmegh plant extract. The method is based on the formation of red-orange colored complex with picric acid in an alkaline medium and obeys beer’s law in the range of 10 - 100µg/mL exhibiting the maximum absorbance at 481nm. The limit of detection and quantification were found to be 1.0µg/mL and 3.0µg/mL, respectively. The calibration curve demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient 0.9998. The Regression equation of the curve was Y=0.0114x + 0.0067. The method was validated according to ICH guidelines.
Andrographolide, Picric acid, NaOH, Validation, ICH guidelines.
A. Suneetha, K.Manasa. Development and Validation of Visible Spectrophotometric Method for Determination of Andrographolide in Kalmegh Plant Extract. Asian J. Pharm. Ana. 4(2): April-June 2014; Page 85-88.
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