Volume No. :   4

Issue No. :  1

Year :  2014

ISSN Print :  2231-5667

ISSN Online :  2231-5675


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Bromatometric Estimation of Cefepime, Cefoperazone, and Cefotriaxone In their Bulk and Dosage Forms



Address:   Abdallah A. El-Shanawany1, Sobhy M. El-Adl1, Lobna M. Abdel-Aziz1, Ali F. Hassan2*
1Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt.
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al –Azhar University, Assuit, Egypt
*Corresponding Author
DOI No:

ABSTRACT:
Two spectrophotometric methods are described for determination of Cefepime, Cefoperasone andCefotriaxone in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbances at 678 nm and 510 nm, respectively. The amount of bromine reacted corresponds to the amount of each drug. Effect of acidity, bromate - bromide volume and reactiontime, on the absorption was studied. Calibration curves were linear over ranges of 1–3 µg.ml-1 for Cefepime,0.4- 1.0 µg.ml-1 for Cefoperazone and 0.3-0.8 µg.ml-1 for Cefotriaxone in case of methylene blue and of 0.05–3.0 µg.ml-1 for Cefepime, 0.75-2.0µg.ml-1 for Cefoperazone and 0.2-1.4 µg.ml-1 for Cefotriaxone in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.
KEYWORDS:
Cefepime, Cefoperazone, Cefotriaxone, Methylene blue and Methyl orange
Cite:
Abdallah A. El-Shanawany, Sobhy M. El-Adl, Lobna M. Abdel-Aziz, Ali F. Hassan. Bromatometric Estimation of Cefepime, Cefoperazone, and Cefotriaxone In their Bulk and Dosage Forms. Asian J. Pharm. Ana. 4(1): Jan.-Mar. 2014; Page 17-27.
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