Volume No. :   3

Issue No. :  1

Year :  2013

ISSN Print :  2231-5667

ISSN Online :  2231-5675


Registration

Allready Registrered
Click to Login

Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Diazepam and Propranolol in Bulk Drug and Its Formulations



Address:   S. J. Daharwal*
University Institute of Pharmacy, Ravishankar Shukla University, Raipur (CG) India-492010
*Corresponding Author
DOI No:

ABSTRACT:
A simple, efficient, precise and accurate Vierodt’s method have been developed for the estimation of Diazepam and Propranolal in pure and in fixed dose Combination. In this method UV spectrum of Diazepam and Propranolal were overlained which involves the formation of Simultaneous equation at maximum wavelength 241 nm and 289 nm. Both the drugs obeyed Beer’s law in the concentration range 2-25 µg/ml and 1-45 µg/ml for Diazepam and Propranolal , respectively. The accuracy of the method was determined by recovery studies and was found to be 100.14±0.219 % and 100.17±0.134 % for Diazepam and Propranolal , respectively. The method was validated as per ICH guidelines. The method was found to be simple, economic, accurate and reproducible and can be used for routine analysis of Diazepam and Propranolal in pure and in fixed dose combination.
KEYWORDS:
Vierodt’s method, Diazepam, Propranolal , ICH guidelines
Cite:
S. J. Daharwal. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Diazepam and Propranolol in Bulk Drug and Its Formulations. Asian J. Pharm. Ana. 3(1): Jan.-Mar. 2013; Page 20-23.
[View HTML]      [View PDF]



Visitor's No. :   102467