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Asian Journal of Pharmaceutical Analysis
ISSN: 2231-5675(Online), 2231-5667(Print)
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Development and validation of a RP-HPLC method for estimation of Thalidomide in solid dosage form
Shiv Kumar Gupta
Pramod Kumar Sharma
Shiv Kumar Gupta*1, Babita Kumar1 and Pramod Kumar Sharma2
1College of Pharmacy, Shree Ganpati Institute of Technology, NH-24, Opp. Jindal Pipes Ltd, Ghaziabad, (U.P.) India.
2School of Pharmacy, Galghotia University, Greater Noida, G B Nagar, (U. P.) India.
A simple, rapid and precise isocratic reverse phase High Performance Liquid Chromatographic method has been developed for the determination of Thalidomide capsule. The separation was achieved with 150 × 4.6mm C18 column using mobile phase as Acetonitrile : Dimethyl Formamide : Water (60:10:30 v/v) at a flow rate of 1.0ml/min with UV detection at 297nm. The retention time of Thalidomide was found to be 2.17min. The linearity range of Thalidomide is 25mg to 200mg with coefficient of correlation 0.9986. The method is found to be accurate, precise and useful in quality control of dosage form.
RP–HPLC, Thalidomide, Method Development and Validation
Shiv Kumar Gupta, Babita Kumar, Pramod Kumar Sharma. Development and validation of a RP-HPLC method for estimation of Thalidomide in solid dosage form. Asian J. Pharm. Ana. 3(1): Jan.-Mar. 2013; Page 17-19.
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